Peach is, obviously, the ultimate femme pick, with the pink gown, the earing and the tiara, submissive in the first games, but totally in control in later games like Mario Kart and Mario Party.
I think Yoshi, that in fact is a dinosaur, is very effeminate and fits the twink category, as it was implied by the post.
OK, I went too far. Lets get back on track. So Luigi was described as "more effeminate than Mario". I think that's true, but I also think that picking Luigi just means you don't want to be so obvious by picking Mario.
And then Mario. This is what was said, “Mario brings out my inner butch. While I consider myself a femme, I just can’t drive around wearing Princess Peach’s godawful pink dress.” I was always Mario.